David Overy

Research Interests:
 
Discovery of novel bioactive molecules from fungal sources.  Contributing as a part of an on going effort to isolate bioactive metabolites from microbes derived from marine samples.  By testing culture fermentation extracts against a variety of antimicrobial and antiproliferative assays, compound purification is carried out by bioassay guided fractionation and subsequent structure elucidation of active compounds.  Current research efforts focused on the application of protein fingerprinting for use as an identification and dereplication tool.

Education:

PhD, Technical University of Denmark, Kgs. Lyngby, Denmark(2005)
MSc(equivalency), Technical University of Denmark, Kgs. Lyngby, Denmark (2001)
BSc, High Honours in Environmental Science, Carleton University, Ottawa, Canada (1999)

Work Experience:

Senior Research Microbiologist, CIBE, Merck Research Laboratories, Merck, Sharp & Dohme de España, Madrid, Spain. (Apr 07 – Oct 2008)
Postdoctoral Researcher, Institute of Biological Sciences, University of Wales, Aberystwyth, UK. (Aug 05 - Apr 07)
Postdoctoral Researcher, Mycology Group, CMB, BioCentrum-DTU, Lyngby, Denmark.
(Apr 05 - Jul 05)
PhD student, Mycology Group, CMB, BioCentrum-DTU, Lyngby, Denmark. (Jan 02 - Apr 05)
Researcher, Dept. of Chemistry, University of Canterbury, Christchurch, New Zealand.
(Oct 03 - May 04)
Researcher/Sole Proprietor, Myco-Botanical Technologies, Oakville, Ontario, Canada.
(Apr 00 - Jan 02)
Diagnostician, Plant Protection Service, Wageningen, Netherlands. (Jul 00 - Nov 00; Mar 01 - Sept 01)
Contract Researcher, ECORC, Agriculture and Agri-Food Canada, Central Experimental Farm, Ottawa, Canada. (June 99 - Mar 00)
Honours Thesis, ECORC, Agriculture and Agri-Food Canada, Ottawa, Canada. (Sept 98 - May 99)
Student Researcher, ECORC, Agriculture and Agri-Food Canada, Ottawa, Canada. (May 98 - Sept. 98)

Publications:

Overy, D.P., Calati, K., Nielsen Kahn, J., Hsu, M., Martin, J., Collado, J., Roemer, T., Harris, G., Parish, C. 2009. Isolation and structure elucidation of parnafungins C and D, isoxazolidinone-containing antifungal natural products. Bioorganic & Medicinal Chemistry Letters, 19: 1224-1227.
 
Bills, G.F., Platas, G., Overy, D.P., Collado, J., Fillola, A., Jimenez, M.R., Martin, J., Gonzalez del Val, A., Vicente, F., Tormo, J.R., Pelaez, F., Calati, K., Harris, G., Parish, C., Xu, D., Roemer, T. 2009. Discovery of the parnafungins, antifungal metabolites that inhibit mRNA polyadenylation, from the Fusarium larvarum complex and other Hypocrealean fungi. Mycologia, 101: 445-468.
 
Bills, G.F., Overy, D.P., Genilloud, O. and Peláez, F. 2009. Contributions of pharmaceutical antibiotic and secondary metabolite discovery to the understanding of microbial defence and antagonism. In: Defensive Mutualism in Microbial Symbiosis. Editors J. White and M.S. Torres, Marcel Dekker, New York. (in press)
 
Overy, D.P., Enot, D.P., Tailliart, K., Jenkins, H., Parker, D., Beckmann, M. and Draper, J. 2008. Explanatory signal interpretation and metabolite identification strategies for nominal mass FIE-MS metabolite fingerprints. Nature Protocols, 3: 471-485.
 
Enot, D.P., Wanchang, L., Beckmann, M., Parker, D., Overy, D.P. and Draper, J. 2008.  Preprocessing, classification modelling and feature selection using flow injection elcetrospray mass spectrometry metabolite fingerprint data. Nature Protocols, 3: 446-470.
 
Parker, D., Beckmann, M., Enot, D.P., Overy, D.P., Caracuel Rios, Z., Gilbert, M., Talbot, N. and Draper, J. 2008.  Rice blast infection of Brachypodium distachyon as a model system to study dynamic host/pathogen interactions. Nature Protocols, 3: 435-445.
 
Beckmann, M., Enot, D.P., Overy, D.P. and Draper, J. 2007. Representation, comparison, and interpretation of metabolome fingerprint data for total composition analysis and quality trait investigation in potato cultivars. Journal of Agricultural and Food Chemistry, 55: 3444-3451.
 
Enot, D.P., Beckmann, M., Overy. D.P., Draper, J. 2006. Predicting interpretability of metabolome models based on behavior, putative identity, and biological relevance of explanatory signals. Proceedings of the National Academy of Science, USA., 103:14865-14870.
 
Overy, D.P., Smedsgaard, J., Frisvad, J.C. and Thrane, U. 2006. Host-derived media used as a predictor for low abundant, in planta metabolite production from necrotrophic fungi.  Journal of Applied Microbiology, 101: 1292-1300.
 
Overy, D.P., Phipps, R., Frydenvang, K. and Larsen,T.O. 2006. epi-Neoxaline, a chemotaxonomic marker for Penicillium tulipae. Biochemical Systematics and Ecology, 34: 345-348.
 
Frisvad, J.C., Larsen, T.O., Dalsgaard, P.W., Lyhne, E.K., Jarvis, B.B., Fettinger, J.C., Seifert, K.A., Louis-seize, G. and Overy, D.P. 2006. Four psychrotolerant species with high chemical diversity consistently producing cycloaspeptide, Penicillium jamesonlandense sp. nov., P. ribeum sp. nov, P. soppii and P. lanosum. International Journal of Systematic and Evolutionary Microbiology, 56: 1427-1437.
 
Valdez, J.G., Makuch, M.A., Ordovini, A.F., Masuelli, R.W., Overy, D.P. and Piccolo,R.J. 2006. Penicillium allii, responsible for blue mold rot in garlic in the Mendoza and San Juan provinces of Argentina. Plant Disease, 55: 583.
 
Valdez, J.G., Makuch, M.A., Ordovini, A.F., Masuelli, R.W., Overy, D.P. and Piccolo,R.J. 2006. First report of Penicillium allii as a field pathogen of garlic (Allium sativum). Plant Pathology, 55: 583.
                            
Overy, D.P. and Frisvad, J.C. 2005. Mycotoxin production and post harvest storage rot of Zingiber officinale by Pencillium brevicompactum. Journal of Food Protection, 68:607-609.
 
Overy, D.P., Frisvad, J.C., Steinmeier, U. and Thrane, U. 2005. Clarification of the agents causing blue mould storage rot upon various flower and vegetable bulbs: implications for mycotoxin contamination. Postharvest Biology and Technology, 35: 217-221.
 
Overy, D.P., Karlshøj, K. and Due, M.J. 2005. Temperature and enzymatic profiling of taxa from the Penicillium series Corymbifera; species responsible for blue mould storage rot in bulbs.  Journal of Plant Pathology, 87: 53-59.
 
Overy, D.P., Larsen, T.O., Dalsgaard, P.W., Frydenvang, K., Phipps, R., Monro, M and Christopherson, C. 2005. Andrastin A and barceloneic metabolites, protein farnesyl transferase inhibitors from Penicillium albocoremium: chemotaxonomic significance and pathological implications. Mycological Research, 11: 1243-1249.
 
Overy, D.P., Nielsen, K.F. and Smedsgaard, J. 2005. Roquefortine/oxaline biosynthesis pathway metabolites in Penicillium ser. Corymbifera: in planta production and implications for competitive fitness. Journal of Chemical Ecology, 31: 2375-2392.
 
Overy, D.P., Valdez, J.G. and Frisvad, J.C. 2005. Revisions to the Penicillium series Corymbifera: agents responsible for blue mold storage rot of various flower and vegetable bulbs.  Canadian Journal of Botany, 83: 1422-1433.
 
Overy, D.P., Zidorn, C.H.W., Petersen, B.O., Duus, J.Ø., Dalsgaard, P.W., Larsen, T.O. and Phipps, R. 2005. Medium dependant production of corymbiferone, a novel metabolite from Penicillium hordei cultured on plant tissue agar. Tetrahedron Letters, 46: 3225-3228.
 
Overy, D.P. and Blunt, J.W. 2004. Corymbiferan lactones from Penicillium hordei: stimulation of novel phenolic metabolites using plant tissue media. Journal of Natural Products, 67: 1850-1853.
 
Overy, D.P. and Frisvad, J.C. 2003. New Penicillium species associated with bulbs and root vegetables. Systematic and Applied Microbiology 26: 631-639.
 
Overy, D.P, Seifert, K., Savard, M.E., Frisvad, J.C. 2003. Spoilage fungi and their mycotoxins in commercially marketed chestnuts. International Journal of Food Microbiology 88: 69-77.

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